Journal: Cell Communication and Signaling : CCS
Article Title: Restoration of Miro1’s N-terminal GTPase function alleviates prenatal stress-induced mitochondrial fission via Drp1 modulation
doi: 10.1186/s12964-025-02172-5
Figure Lengend Snippet: Reduced intra-mitochondrial Ca 2+ contributed to Drp1-mediated mitochondrial fission. ( A , C , E ) Human NSCs were cultured in neuronal differentiation media for 6 days and treated with cortisol (1 µM) for 24 h. Furthermore, human NSCs were transduced with control vector, Miro1 WT , or Miro1 P13V expression vector 24 h after seeding. ( A – C ) Human NSCs were immunostained with mitotracker Red (MTR, red). ( A ) Quantification of mitochondrial shape descriptors (form factor and aspect ratio) were determined by using Fiji software. Scale bars, 20 μm. n = 4. ( B ) Human NSCs were treated with SB202190 (10 µM) or Ru 360 (10 µM) 30 min before cortisol treatment. n = 5. ( C ) Human NSCs were transfected with NT siRNA or DNM1L siRNA 24 h before cortisol treatment. n = 5. The expressions of Drp1 were determined by western blot. The β-actin was used as a loading control. ( D ) Human NSCs were cultured in neuronal differentiation media for 6 days and treated with SB 202,190 or ionomycin (10 µM) for 30 min prior to cortisol treatment. The expressions of p-Drp1 (Ser616) and Drp1 were determined by western blot. The β-actin was used as a loading control. n = 5. ( E ) The expressions of p-Drp1 and Drp1 were determined by western blot. The β-actin was used as a loading control. n = 5. Quantitative data are presented as a mean ± S.E.M. The representative images were acquired by SRRF imaging system. *, ** indicates p < 0.05, p < 0.01 respectively. ns means not significant
Article Snippet: The siRNA specific for human DNM1L and mouse dnm1l , and siRNA specific for nontargeting (NT) were purchased from Bioneer Corporation (Daejeon, Korea).
Techniques: Cell Culture, Transduction, Control, Plasmid Preparation, Expressing, Software, Transfection, Western Blot, Imaging
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